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Specialist in fertility treatments and gynaecology

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Individual attention for each mare for optimal fertility

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OPU, ICSI & ET

WHO WE ARE

SPECIALIST VETERINARY EQUINE REPRODUCTIVE SERVICES

EQUICEPTION: OUR VISION

To become a leading Equine Fertility Clinic – a Centre of Excellence – focussed on client satisfaction through the provision of a high quality results based service.

“How can we achieve this? Understanding that mares can present differently, it is our challenge to find the right approach for each horse. We combine sound theoretical knowledge with extensive clinical experience to tailor an approach to get the best results from your mare. Our approach to our clients is personal and focused, building working relationships and confidence, this is important to us….”

Dr. Patrick Brogan & Jaimy Oude-Wesselink – Directors Equiception B.V.

WHO WE ARE

SPECIALIST VETERINARY EQUINE REPRODUCTIVE SERVICES

EQUICEPTION: OUR VISION

To become a leading Equine Fertility Clinic – a Centre of Excellence – focussed on client satisfaction through the provision of a high quality results based service.

“How can we achieve this? Understanding that mares can present differently, it is our challenge to find the right approach for each horse. We combine sound theoretical knowledge with extensive clinical experience to tailor an approach to get the best results from your mare. Our approach to our clients is personal and focused, building working relationships and confidence, this is important to us….”

Dr. Patrick Brogan & Jaimy Oude-Wesselink – Directors Equiception B.V.

WHAT WE DO

OUR EXPERTISE: AT A GLANCE

ROUTINE BREEDING MANAGEMENT OF MARES

Breeding management of mares is optimised whilst under care at our clinic. Chilled and frozen semen breeding – many stallions available.

Read more

FERTILITY TREATMENTS – PROBLEMATIC MARES

Equiception offers intensive, gynaecological treatments and therapies for fertility problems and problematic pregnancies in mares.

Read more

OVUM PICK UP (OPU) & INTRACYTOPLASMIC SPERM INJECTION (ICSI)

OPU & ICSI are two different things but are inextricably linked, ICSI always follows the OPU. OPU is performed on Mondays and Tuesdays from September through to April.

Read more

Avantea website

EMBRYO TRANSFER & RECIPIENT MARE MANAGEMENT

With our mobile laboratory, we can wash and transplant fresh embryos on flushing location. We have our own recipient mares and can bring embryos to Equiception for transfer. Client owned recipient mares to receive frozen ICSI embryos can also be managed at our clinical facility for embryo transfer.

Read more about embryo transfer.

Read more about recipient mare management

WHAT WE DO

OUR EXPERTISE: AT A GLANCE

ROUTINE BREEDING MANAGEMENT OF MARES

Breeding management of mares is optimised whilst under care at our clinic. Chilled and frozen semen breeding – many stallions available. Read more

FERTILITY TREATMENTS – PROBLEMATIC MARES

Equiception offers intensive, gynaecological treatments and therapies for fertility problems and problematic pregnancies in mares. Read more

OPU & ICSI

OPU & ICSI are two different things but are inextricably linked, ICSI always follows the OPU. OPU is performed on Mondays and Tuesdays from September through to April. Read more
Avantea website

EMBRYO TRANSFER & RECIPIENT MARE MANAGEMENT

With our mobile laboratory, we can wash and transplant fresh embryos on flushing location. We have our own recipient mares and can bring embryos to Equiception for transfer. Client owned recipient mares to receive frozen ICSI embryos can also be managed at our clinical facility for embryo transfer. Read more

ROUTINE BREEDING MANAGEMENT OF MARES

24 HOURS CUSTOMIZED CARE

At Equiception we have our own private clinic for the cycle and fertility management of our clients’ mares. Here, amongst other things, inseminations and pregnancy checks occur. Our clinic offers all the benefits of 24-hour care to deliver a customized service and respond accurately to the cycle of the mare. The well-being of mares in our care is our priority; as a healthy and settled mare responds positively with improved fertility. Mares for breeding can stay with us for the short-term.

ROUTINE BREEDING MANAGEMENT OF MARES

24 HOURS CUSTOMIZED CARE

At Equiception we have our own private clinic for the cycle and fertility management of our clients’ mares. Here, amongst other things, inseminations and pregnancy checks occur. Our clinic offers all the benefits of 24-hour care to deliver a customized service and respond accurately to the cycle of the mare. The well-being of mares in our care is our priority; as a healthy and settled mare responds positively with improved fertility. Mares for breeding can stay with us for the short-term.

FERTILITY TREATMENTS – PROBLEMATIC MARES

FERTILITY SPECIALISTS

Equiception specializes in intensive reproductive treatments and therapies to manage sub-fertility. We identify and investigate uterine inflammation and infections and can treat uterine cysts. Amongst other things, Patricks’ expertise focuses on the surgical repair of the cervix, vagina and the removal of scar tissue that can hinder fertility.

Personal attention from a professional commitment is our priority, with a focus to the continued health and recovery of your mare. Then consulting with you the best way forward to maintain the fertility of your mare.

FERTILITY TREATMENTS – PROBLEMATIC MARES

FERTILITY SPECIALISTS

Equiception specializes in intensive reproductive treatments and therapies to manage sub-fertility. We identify and investigate uterine inflammation and infections and can treat uterine cysts. Amongst other things, Patricks’ expertise focuses on the surgical repair of the cervix, vagina and the removal of scar tissue that can hinder fertility.

Personal attention from a professional commitment is our priority, with a focus to the continued health and recovery of your mare. Then consulting with you the best way forward to maintain the fertility of your mare.

OPU & ICSI

CONSISTENT RESULTS, SAFE, WITH A FOCUS ON WELFARE FOR THE HORSE

OPU and ICSI are two different things, but are inextricably linked.Hetéén volgt dus altijd op het ander, maarrepresenteren de verschillende stappen in hetcompleteproces.The one always follows the other, but both represent the different steps of the complete process.

OPU can actually be seen as the first step, in which the follicles are punctured, scraped and flushed to recover the oocytes (egg cells). To do this as safely as possible, the mare is sedated and taken to our OPU clinical treatment area. Further medication (anti-inflammatories, pre-procedural antibiotics and an intestinal relaxant) is administered and most mares also receive an epidural so that the procedure is as safe and pain-free as possible. At this time a catheter is placed in the bladder and the perineal region is thoroughly cleaned with a mild anti-septic soap.

A specialised vaginal ultrasound probe, with a needle guide attached, is inserted cranially into the vagina and placed to the left or right of the cervix. At this time the veterinarian performing the procedure inserts his or her arm rectally and locates the right ovary (if the probe is positioned to right of cervix) or left ovary (if the probe is positioned to left of the cervix). The ovary is then flipped medially and retracted caudally to communicate over the end of the vaginal probe, the wall of the vaginal vault separates the two. Follicles are then visualized and the procedure can commence. Each follicle larger than 1 cm in diameter is punctured, drained, scraped and flushed several times. The fluid is collected in a warm sterile glass bottle, and once full it is taken directly to our laboratory to be filtered and examined for the presence of the oocytes. The oocytes are ‘washed’, assessed and packaged for transport to the laboratory.

ICSI is the technique where a single sperm cell is injected into a matured oocyte. This is actually an advanced form of IVF; the ‘normal’ IVF whereby sperm and oocytes are placed together in a dish, is unsuccessful in horses because the sperm cell is unable to penetrate the oocyte in current laboratory culturing conditions. To date, we have sent the oocytes to Avantea, a laboratory for advanced technologies in animal reproduction in Italy, where they perform the ICSI, embryo culture and freezing. This is very specialized work with every small detail having an influence on the results. After successful fertilisation of an oocyte, the early embryo develops for 7-9 days in the laboratory. Cultured embryos of good quality are then frozen. The success of the freezing of ICSI-produced embryos lies in the delayed development of embryos produced in the laboratory, although 7-9 days have passed since fertilisation they are the equivalent (developmentally) with day 5 embryos.

Read more

FAQ

Contact us for semen availability for use in ICSI

OPU_Filler

OPU & ICSI

CONSISTENT RESULTS, SAFE, WITH A FOCUS ON WELFARE FOR THE HORSE

OPU and ICSI are two different things, but are inextricably linked.Hetéén volgt dus altijd op het ander, maarrepresenteren de verschillende stappen in hetcompleteproces.The one always follows the other, but both represent the different steps of the complete process.

OPU can actually be seen as the first step, in which the follicles are punctured, scraped and flushed to recover the oocytes (egg cells). To do this as safely as possible, the mare is sedated and taken to our OPU clinical treatment area. Further medication (anti-inflammatories, pre-procedural antibiotics and an intestinal relaxant) is administered and most mares also receive an epidural so that the procedure is as safe and pain-free as possible. At this time a catheter is placed in the bladder and the perineal region is thoroughly cleaned with a mild anti-septic soap.

A specialised vaginal ultrasound probe, with a needle guide attached, is inserted cranially into the vagina and placed to the left or right of the cervix. At this time the veterinarian performing the procedure inserts his or her arm rectally and locates the right ovary (if the probe is positioned to right of cervix) or left ovary (if the probe is positioned to left of the cervix). The ovary is then flipped medially and retracted caudally to communicate over the end of the vaginal probe, the wall of the vaginal vault separates the two. Follicles are then visualized and the procedure can commence. Each follicle larger than 1 cm in diameter is punctured, drained, scraped and flushed several times. The fluid is collected in a warm sterile glass bottle, and once full it is taken directly to our laboratory to be filtered and examined for the presence of the oocytes. The oocytes are ‘washed’, assessed and packaged for transport to the laboratory.

ICSI is the technique where a single sperm cell is injected into a matured oocyte. This is actually an advanced form of IVF; the ‘normal’ IVF whereby sperm and oocytes are placed together in a dish, is unsuccessful in horses because the sperm cell is unable to penetrate the oocyte in current laboratory culturing conditions. To date, we have sent the oocytes to Avantea, a laboratory for advanced technologies in animal reproduction in Italy, where they perform the ICSI, embryo culture and freezing. This is very specialized work with every small detail having an influence on the results. After successful fertilisation of an oocyte, the early embryo develops for 7-9 days in the laboratory. Cultured embryos of good quality are then frozen. The success of the freezing of ICSI-produced embryos lies in the delayed development of embryos produced in the laboratory, although 7-9 days have passed since fertilisation they are the equivalent (developmentally) with day 5 embryos.

Read more

FAQ

Contact us for semen availability for use in ICSI

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EMBRYO TRANSFER & recipient mare management

EXPERIENCED, PROFESSIONAL AND INVOLVED

Embryo transfer (ET) refers to the removal of an embryo from the uterus of one mare (donor mare) and the transfer of that embryo into the uterus of another mare (recipient mare).

The preovulatory follicle of the donor mare is monitored via ultrasonography per rectum by the managing veterinarian. The type of semen used (fresh versus frozen), determines whether the mare is inseminated when the follicle appears to be close to ovulation (fresh semen), or immediately after ovulation has taken place (frozen semen). Appropriate timing of insemination is commonly facilitated by induction of ovulation by administration of medication. If bred prior to ovulation, the preovulatory follicle is monitored daily after insemination to determine the exact day of ovulation (day 0). Typically, on day 7 or 8 after ovulation the embryo is flushed from the donor mare’s uterus. Flushing is performed by repeatedly filling the uterus with approximately 1 litre of flush solution and then draining the fluid through an embryo filter. Fluid retained in the filter is then examined under a microscope, and if present, the embryo is identified and graded for quality. After the embryo is washed it is transferred trans-cervically into the uterus of a recipient mare (surrogate) that ovulated from one day before up to 4 days after the donor mare. The recipient mare will be checked for pregnancy approximately a week later to determine if the embryo transfer was successful.

At Equiception it is expected of a fertile donor mare, inseminated with an appropriate dose of fertile semen, to deliver an embryo in 75% of cases with the expected pregnancy rate after transfer in the recipient being about 85%, together resulting in approximately a 65% pregnancy rate per cycle.

Obviously, when dealing with sub-fertile and/or older donor mares or semen of poor quality these rates will decrease, therefore it is important to set realistic expectations.

Read more on Embryo Transfer (Mare Owners)

Donor Mare Management (Veterinary Information)

Recipient Mare Management (Veterinary Information)

FAQ

EMBRYO TRANSFER & recipient mare management

EXPERIENCED, PROFESSIONAL AND INVOLVED

Embryo transfer (ET) refers to the removal of an embryo from the uterus of one mare (donor mare) and the transfer of that embryo into the uterus of another mare (recipient mare).

The preovulatory follicle of the donor mare is monitored via ultrasonography per rectum by the managing veterinarian. The type of semen used (fresh versus frozen), determines whether the mare is inseminated when the follicle appears to be close to ovulation (fresh semen), or immediately after ovulation has taken place (frozen semen). Appropriate timing of insemination is commonly facilitated by induction of ovulation by administration of medication. If bred prior to ovulation, the preovulatory follicle is monitored daily after insemination to determine the exact day of ovulation (day 0). Typically, on day 7 or 8 after ovulation the embryo is flushed from the donor mare’s uterus. Flushing is performed by repeatedly filling the uterus with approximately 1 litre of flush solution and then draining the fluid through an embryo filter. Fluid retained in the filter is then examined under a microscope, and if present, the embryo is identified and graded for quality. After the embryo is washed it is transferred trans-cervically into the uterus of a recipient mare (surrogate) that ovulated from one day before up to 4 days after the donor mare. The recipient mare will be checked for pregnancy approximately a week later to determine if the embryo transfer was successful.

At Equiception it is expected of a fertile donor mare, inseminated with an appropriate dose of fertile semen, to deliver an embryo in 75% of cases with the expected pregnancy rate after transfer in the recipient being about 85%, together resulting in approximately a 65% pregnancy rate per cycle.

Obviously, when dealing with sub-fertile and/or older donor mares or semen of poor quality these rates will decrease, therefore it is important to set realistic expectations.

Read more on Embryo Transfer (Mare Owners)

Donor Mare Management (Veterinary Information)

Recipient Mare Management (Veterinary Information)

FAQ

Our team

INTRODUCING

profiel-patrick

Drs. Patrick Brogan
Director, Veterinarian and equine reproduction specialist.

Driven, passionate and with a strong focus on improving and developing treatments for sub-fertility.

CV

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Dhr. Jaimy Oude-Wesselink
Director, clinical manager

Calm and personal, a man with integrity. Passionate about horse jumping. Thorough knowledge of marepedigrees and studs, and connections in the industry.

CV

profiel-jorg

Dhr. Jorg Kivits
Clinical assistant and stable manager.

Anna-Lotte Bos

Drs. Anna-Lottte Bos
Associate Veterinarian, student management

Motivated and dedicated, keeping the high standard in horsereproduction we at Equiception want to pursue.

CV

Elèna Giampieri

Drs. Eléna Giampieri
Associate Veterinarian

Calm, independant and driven to achieve excellence in horsereproduction.

CV

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Mw. Mariël Oude-Wesselink
Microbiologist, Fertility laboratory

Larice Wijsman

Larice Wijsman 
Equine Carer

Ashley Hovestad

Ashley Hovestad
Equine Carer

Say hello

We’re happy to visit

ET appointmentMare managementOPU/ICSI appointmentICSI embryo transfer

Address

Equiception
Milandweg 69
3474 KK Zegveld
The Netherlands

 

contact-smartphone

Patrick: +31 (0) 657450757
Jaimy: +31 (0) 653583247

 

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Privacyverklaring

Say hello

We’re happy to visit

ET appointmentMare managementOPU/ICSI appointmentICSI embryo transfer

Address

Equiception
Milandweg 69
3474 KK Zegveld
Nederland